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Ot007 10x tbs with tween 20 biosolution materials tbs buffer ph 7 4 o 10x dilute to 1x for ihc 80g nacl 2g kcl 30g tris base solution 1 dissolve tbs buffer tris buffered saline 10x solution ph 7 4 canvax tris buffered saline tbs 10x cell signaling technology